The enzymes used to produce invert sugar syrups, i.e. ß-fructofuranosidase (EC 3.2.1.26),
ß-amylase (EC 3.2.1.2) and glucoamylase (��-amylase, EC 3.2.1.3), do not occur naturally in
honey (foreign enzymes). Therefore, adulteration with the foreign enzymes and the sugar
syrup produced with them, respectively, can be directly detected by the specific analytical
determination of these foreign enzymes serving as marker substances. Suitable analytical methods (Fig. 2) were already developed and validated [6, 7] for ßfructofuranosidase
(ßFF).